RT Journal Article
SR Electronic
T1 Evidence for a Medium-Chain Fatty Acid:Coenzyme A Ligase (Adenosine Monophosphate) That Activates Salicylate
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 260
OP 268
VO 7
IS 3
A1 PAUL G. KILLENBERG
A1 EUGENE D. DAVIDSON
A1 LESLIE T. WEBSTER, JR.
YR 1971
UL http://molpharm.aspetjournals.org/content/7/3/260.abstract
AB A medium-chain fatty acid:CoA ligase (AMP) which activates salicylate has been purified approximately 30-fold from bovine liver "mitochondria." The new enzyme is not completely free of a medium-chain fatty acid:CoA ligase (AMP) (EC 6.2.1.2) of the type first described by Mahler, Wakil, and Bock [J. Biol. Chem. 204, 453 (1953)], but the two enzymes can be distinguished readily from each other. The enzyme with salicyl-CoA synthetase activity is more easily inactivated, migrates more slowly toward the anode during disc gel electrophoresis, and utilizes a wider spectrum of aromatic acid substrates than the enzyme described earlier. Evidence was obtained that both enzymes could catalyze the activation of hexanoate, benzoate, o-methoxybenzoate, and anthranilate, but only the "salicylate" enzyme displayed activity with salicylate and p-aminosalicylate. ACKNOWLEDGMENT The authors wish to thank Dr. Sung Ling Yuan for his valuable technical assistance.