PT  - JOURNAL ARTICLE
AU  - F. MITANI
AU  - A. P. ALVARES
AU  - S. SASSA
AU  - A. KAPPAS
TI  - Preparation and Properties of a Solubilized Form of Cytochrome P-450 from Chick Embryo Liver Microsomes
DP  - 1971 May 01
TA  - Molecular Pharmacology
PG  - 280--292
VI  - 7
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/7/3/280.short
4100  - http://molpharm.aspetjournals.org/content/7/3/280.full
SO  - Mol Pharmacol1971 May 01; 7
AB  - A procedure for the partial purification and subsequent solubilization of cytochrome P-450 from microsomal membranes of chick embryo liver is described. Successive treatment of microsomes with Protease VII and Lubrol WX yielded a solubilized preparation with about a 1.8-fold increase in the specific content of cytochrome P-450, measured as millimicromoles per milligram of protein, with no detectable amounts of cytochrome b5 and minimal activities of NADPH- and NADH-cytochrome c reductases. The CO difference spectrum of the solubilized cytochrome P-450 showed an absence of cytochrome P-420; however, the absolute spectrum of the CO complex of the reduced preparation displayed a peak at 420 mµ. The solubilized cytochrome P-450 obtained from chick embryo livers interacted with various substrates to give type I or type II spectra similar to those observed in other species. Cytochrome P-450 in chick embryo liver was inducible by drugs, polycyclic hydrocarbons, and steroids.