PT - JOURNAL ARTICLE AU - Ibolya E. András AU - GeunBae Rha AU - Wen Huang AU - SungYong Eum AU - Pierre-Olivier Couraud AU - Ignacio A. Romero AU - Bernhard Hennig AU - Michal Toborek TI - Simvastatin Protects against Amyloid β and HIV-1 Tat-Induced Promoter Activities of Inflammatory Genes in Brain Endothelial Cells AID - 10.1124/mol.107.042028 DP - 2008 May 01 TA - Molecular Pharmacology PG - 1424--1433 VI - 73 IP - 5 4099 - http://molpharm.aspetjournals.org/content/73/5/1424.short 4100 - http://molpharm.aspetjournals.org/content/73/5/1424.full SO - Mol Pharmacol2008 May 01; 73 AB - Increased deposition of amyloid β (Aβ) is characteristic for normal aging and human immunodeficiency virus-1 (HIV-1)-associated alterations of the central nervous system. In addition, both Aβ and HIV-1 are known to induce cellular oxidative stress and disruption of the blood-brain barrier (BBB). Therefore, we hypothesize that Aβ and HIV-1 protein Tat can potentiate their proinflammatory effects at the brain endothelium level. To address this hypothesis, we studied promoter activity of three proinflammatory genes in an in vitro BBB model of human brain microvascular endothelial cells (HBMEC) cocultured with a human astrocyte cell line producing Tat (SVGA-Tat cells) and exposed to Aβ. Treatment of HBMEC with Aβ(1-40) in the presence of SVGA-Tat cells resulted in a significant up-regulation of E-selectin, CC chemokine ligand-2, and interleukin-6 promoter activities and protein levels compared with the individual effects of Aβ or Tat. In addition, Aβ markedly amplified E-selectin promoter activity in HBMEC cocultured with HIV-1-infected Jurkat T cells. Simvastatin, the 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, effectively blocked proinflammatory reactions induced by Aβ in cocultures with SVGA-Tat cells or with HIV-1-infected Jurkat cells. The present study indicates that a combined exposure to Aβ and Tat or HIV-1 can synergistically potentiate the expression of inflammatory genes in brain endothelial cells. In addition, simvastatin may provide a beneficial influence by reducing these effects at the BBB level.