TY - JOUR T1 - Canonical Transient Receptor Potential Channel (TRPC) 3 and TRPC6 Associate with Large-Conductance Ca<sup>2+</sup>-Activated K<sup>+</sup> (BK<sub>Ca</sub>) Channels: Role in BK<sub>Ca</sub> Trafficking to the Surface of Cultured Podocytes JF - Molecular Pharmacology JO - Mol Pharmacol SP - 466 LP - 477 DO - 10.1124/mol.108.051912 VL - 75 IS - 3 AU - Eun Young Kim AU - Claudia P. Alvarez-Baron AU - Stuart E. Dryer Y1 - 2009/03/01 UR - http://molpharm.aspetjournals.org/content/75/3/466.abstract N2 - Large-conductance (BKCa type) Ca2+-activated K+ channels encoded by the Slo1 gene and various canonical transient receptor potential channels (TRPCs) are coexpressed in many cell types, including podocytes (visceral epithelial cells) of the renal glomerulus. In this study, we show by coimmunoprecipitation and GST pull-down assays that BKCa channels can associate with endogenous TRPC3 and TRPC6 channels in differentiated cells of a podocyte cell line. Both types of TRPC channels colocalize with Slo1 in podocytes and in human embryonic kidney (HEK) 293T cells transiently coexpressing the TRPC channels with Slo1. In HEK293T cells, coexpression of TRPC6 increased surface expression of a Slo1 subunit splice variant (Slo1VEDEC) that is typically retained in intracellular compartments, as assessed by cell-surface biotinylation assays and confocal microscopy. Corresponding currents through BKCa channels were also increased with TRPC6 coexpression, as assessed by whole-cell and excised inside-out patch recordings. By contrast, coexpression of TRPC3 had no effect on the surface expression of BKCa channels in HEK293T cells or on the amplitudes of currents in whole cells or excised patches. In podocytes, small interfering RNA knockdown of endogenous TRPC6 reduced steady-state surface expression of endogenous Slo1 channels, but knockdown of TRPC3 had no effect. TRPC6, but not TRPC3 knockdown also reduced voltage-evoked outward current through podocyte BKCa channels. These data indicate that TRPC6 and TRPC3 channels can bind to Slo1, and this colocalization may allow them to serve as a source of Ca2+ for the activation of BKCa channels. TRPC6 channels also play a role in the regulation of surface expression of a subset of podocyte BKCa channels. The American Society for Pharmacology and Experimental Therapeutics ER -