PT  - JOURNAL ARTICLE
AU  - Mohammed M. A. Safhi
AU  - Claire Rutherford
AU  - Catherine Ledent
AU  - William A. Sands
AU  - Timothy M. Palmer
TI  - Priming of Signal Transducer and Activator of Transcription Proteins for Cytokine-Triggered Polyubiquitylation and Degradation by the A<sub>2A</sub> Adenosine Receptor
AID  - 10.1124/mol.109.062455
DP  - 2010 Jun 01
TA  - Molecular Pharmacology
PG  - 968--978
VI  - 77
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/77/6/968.short
4100  - http://molpharm.aspetjournals.org/content/77/6/968.full
SO  - Mol Pharmacol2010 Jun 01; 77
AB  - Here we demonstrate that overexpression of the human A2A adenosine receptor (A2AAR) in vascular endothelial cells confers an ability of interferon-α and a soluble IL-6 receptor/IL-6 (sIL-6Rα/IL-6) trans-signaling complex to trigger the down-regulation of signal transducer and activator of transcription (STAT) proteins. It is noteworthy that STAT down-regulation could be reversed by coincubation with A2AAR-selective inverse agonist 4-(2-[7-amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol (ZM241385) but not adenosine deaminase, suggesting that constitutive activation of the receptor was responsible for the effect. Moreover, STAT down-regulation was selectively abolished by proteasome inhibitor N-benzoyloxycarbonyl (Z)-Leu-Leu-leucinal (MG132), whereas lysosome inhibitor chloroquine was without effect. Down-regulation required Janus kinase (JAK) activity and a Tyr705→Phe-mutated STAT3 was resistant to the phenomenon, suggesting that JAK-mediated phosphorylation of this residue is required. Consistent with this hypothesis, treatment of A2AAR-overexpressing cells with sIL-6Rα/IL-6 triggered the accumulation of polyubiquitylated wild-type but not Tyr705→Phe-mutated STAT3. Support for a functional role of this process was provided by the observation that A2AAR overexpression attenuated the JAK/STAT-dependent up-regulation of vascular endothelial growth factor receptor-2 by sIL-6Rα/IL-6. Consistent with a role for endogenous A2AARs in regulating STAT protein levels, prolonged exposure of endogenous A2AARs in endothelial cells with ZM241385 in vitro triggered the up-regulation of STAT3, whereas deletion of the A2AAR in vivo potentiated STAT1 expression and phosphorylation. Together, these experiments support a model whereby the A2AAR can prime JAK-phosphorylated STATs for polyubiquitylation and proteasomal degradation and represents a new mechanism by which an anti-inflammatory seven-transmembrane receptor can negatively regulate JAK/STAT signaling.Copyright © 2010 The American Society for Pharmacology and Experimental Therapeutics