TY - JOUR T1 - Production of Antibodies to Rat Liver Phenylalanine Hydroxylase Cross-reactivity with Other Pterin-Dependent Hydroxylases JF - Molecular Pharmacology JO - Mol Pharmacol SP - 501 LP - 510 VL - 8 IS - 5 AU - PAUL A. FRIEDMAN AU - TOM LLOYD AU - SEYMOUR KAUFMAN Y1 - 1972/09/01 UR - http://molpharm.aspetjournals.org/content/8/5/501.abstract N2 - A potent, specific antiserum to rat liver phenylalanine hydroxylase was produced in sheep. Agar double-immunodiffusion reaction and enzyme inhibition studies revealed extensive cross-reactivity between the antiserum and phenylalanine hydroxylases from guinea pig, mouse, monkey, and human liver, as well as from rat kidney. No cross-reactivity with Pseudomonas phenylalanine hydroxylase could be demonstrated. Further studies suggested cross-reactivity with bovine adrenal and rat brain tyrosine hydroxylases, but not with rat brain tryptophan hydroxylase, rat kidney aromatic L-amino acid decarboxylase, or bovine adrenal dopamine β-hydroxylase. ER -