PT  - JOURNAL ARTICLE
AU  - ROLAND D. CIARANELLO
AU  - HANS J. DANKERS
AU  - JACK D. BARCHAS
TI  - The Enzymatic Formation of Methanol from <em>S</em>-Adenosylmethionine by Various Tissues of the Rat
DP  - 1972 May 01
TA  - Molecular Pharmacology
PG  - 311--317
VI  - 8
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/8/3/311.short
4100  - http://molpharm.aspetjournals.org/content/8/3/311.full
SO  - Mol Pharmacol1972 May 01; 8
AB  - An enzyme which forms methanol from S-adenosylmethionine is demonstrated to be present in a variety of mammalian tissues. That one of the products is methanol was determined by two means: (a) crystallization as [methyl]dinitrobenzoate, and (b) synthesis of 35S-labeled S-adenosylmethionine and demonstration that 35S was not incorporated into the volatile product. Highest levels of enzyme activity are found in the pituitary and hypothalamus. Other areas which contain the enzyme include brain, kidney, liver, lung, and heart. The enzyme from brain has a Km of 2.5 µM for S-adenosylmethionine. Enzyme activity is found predominantly in the supernatant fraction of brain homogenates.