PT  - JOURNAL ARTICLE
AU  - ALFRED G. GILMAN
AU  - BRUCE K. SCHRIER
TI  - Adenosine Cyclic 3',5'-Monophosphate in Fetal Rat Brain Cell Cultures
DP  - 1972 Jul 01
TA  - Molecular Pharmacology
PG  - 410--416
VI  - 8
IP  - 4
4099  - http://molpharm.aspetjournals.org/content/8/4/410.short
4100  - http://molpharm.aspetjournals.org/content/8/4/410.full
SO  - Mol Pharmacol1972 Jul 01; 8
AB  - The effects of various neurohormones and other chemical agents on intracellular levels of adenosine cyclic 3',5'-monophosphate were evaluated in surface cultures of fetal rat brain cells. Treatment of cells with dopamine, prostaglandin F2α ,5-hydroxytryptamine, or KCl was without effect on cyclic AMP levels. Increases were found following exposure to adenosine, prostaglandin E1 , norepinephrine, or isoproterenol. The catecholamines were considerably more effective than the other compounds, causing up to a 100-fold increase in cyclic AMP content. This maximal response was not affected by 1 mM theophylline, was blocked by the beta adrenergic antagonists sotalol and dichloroisoproterenol, and was maximal after 5-15 min of exposure of the cells to the catecholamine. The response to isoproterenol developed during culture; its magnitude increased to a maximum after about 14 days in culture. ACKNOWLEDGMENTS We thank Miss Eve Cutler for her excellent technical assistance, Dr. John Pike of the Upjohn Company for prostaglandins, and the Mead Johnson Company for sotalol.