PT  - JOURNAL ARTICLE
AU  - Hsiao-Sheng Liu
AU  - Ching-Shiun Ke
AU  - Hung-Chi Cheng
AU  - Chi-Ying F. Huang
AU  - Chun-Li Su
TI  - Curcumin-Induced Mitotic Spindle Defect and Cell Cycle Arrest in Human Bladder Cancer Cells Occurs Partly through Inhibition of Aurora A
AID  - 10.1124/mol.111.072512
DP  - 2011 Oct 01
TA  - Molecular Pharmacology
PG  - 638--646
VI  - 80
IP  - 4
4099  - http://molpharm.aspetjournals.org/content/80/4/638.short
4100  - http://molpharm.aspetjournals.org/content/80/4/638.full
SO  - Mol Pharmacol2011 Oct 01; 80
AB  - Curcumin, an active compound in turmeric and curry, has been proven to induce tumor apoptosis and inhibit tumor proliferation, invasion, angiogenesis, and metastasis via modulating numerous targets in various types of cancer cells. Aurora A is a mitosis-related serine-threonine kinase and plays important roles in diverse human cancers. However, the effect of curcumin on Aurora A has not been reported. In this study, Aurora A promoter activity and mRNA expression were inhibited in curcumin-treated human bladder cancer T24 cells, suggesting that Aurora A is regulated at the transcription level. We also found that curcumin preferentially inhibited the growth of T24 cells, which show a higher proliferation rate, invasion activity, and expression level of Aurora A compared with that of human immortalized uroepithelial E7cells. Furthermore, inhibition of phosphorylation of Aurora A and its downstream target histone H3 accompanied by the formation of monopolar spindle, induction of G2/M phase arrest, and reduction in cell division in response to curcumin were detected in T24 cells. These curcumin-induced phenomena were similar to those using Aurora A small interfering RNA and were attenuated by ectopic expression of Aurora A. Therefore, the antitumor mechanism of curcumin is Aurora A-related, which further supports the application of curcumin in treatments of human cancers.