PT  - JOURNAL ARTICLE
AU  - Xin-Ming Ma
AU  - Jian-ping Huang
AU  - Xiaonan Xin
AU  - Yan Yan
AU  - Richard E. Mains
AU  - Betty A. Eipper
TI  - A Role for Kalirin in the Response of Rat Medium Spiny Neurons to Cocaine
AID  - 10.1124/mol.112.080044
DP  - 2012 Oct 01
TA  - Molecular Pharmacology
PG  - 738--745
VI  - 82
IP  - 4
4099  - http://molpharm.aspetjournals.org/content/82/4/738.short
4100  - http://molpharm.aspetjournals.org/content/82/4/738.full
SO  - Mol Pharmacol2012 Oct 01; 82
AB  - Kalirin-7 (Kal7), the major kalirin isoform in adult brain, plays a key role in the formation of dendritic spines in hippocampal/cortical neurons. Its role in the GABAergic medium spiny neurons (MSNs) of the nucleus accumbens (NAc) and striatum, the areas known to play a key role in the common reward pathway, is not as well understood. Although Kal7 expression in mouse NAc increased in response to cocaine, MSN dendritic spine density did not differ from that for the wild type in Kal7-null mice. Unlike wild-type mice, Kal7-null mice did not respond to cocaine with an increase in MSN dendritic spine density. To explore further the role of Kal7 in cocaine-induced alterations in MSN morphology, we turned to the rat. Based on immunostaining, both Kal7 and Kal12 are expressed at moderate levels in the MSNs of the NAc and striatum. Expression of Kal7 and Kal12 in MSNs of both areas increases after repeated cocaine treatments. Overexpression of Kal7 in cultured MSN neurons increases dendritic spine density, as observed in rats after long-term cocaine administration. Reducing endogenous expression of all major kalirin isoforms in cultured MSN neurons causes a decrease in total dendritic length and dendritic spine density. These data suggest that kalirin is essential for maintaining spine density in NAc MSNs under normal conditions and that Kal7 is an obligatory intermediate in the response of MSNs to repeated exposure to cocaine.