RT Journal Article
SR Electronic
T1 Metformin Restores Intermediate-Conductance Calcium-Activated K<sup>+</sup> Channel– and Small-Conductance Calcium-Activated K<sup>+</sup> Channel–Mediated Vasodilatation Impaired by Advanced Glycation End Products in Rat Mesenteric Artery
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 580
OP 591
DO 10.1124/mol.114.092874
VO 86
IS 5
A1 Zhao, Li-Mei
A1 Wang, Yan
A1 Yang, Yong
A1 Guo, Rong
A1 Wang, Nan-Ping
A1 Deng, Xiu-Ling
YR 2014
UL http://molpharm.aspetjournals.org/content/86/5/580.abstract
AB The present study was designed to investigate the effect of metformin on the impairment of intermediate-conductance and small-conductance Ca2+-activated potassium channels (IKCa and SKCa)–mediated relaxation in diabetes and the underlying mechanism. The endothelial vasodilatation function of mesenteric arteries was assessed with the use of wire myography. Expression levels of IKCa and SKCa and phosphorylated Thr172 of AMP-activated protein kinase (AMPK) were measured using Western blot technology. The channel activity was observed using a whole-cell patch voltage clamp. Reactive oxygen species (ROS) were measured using dihydroethidium and 2′,7′-dichlorofluorescein diacetate. Metformin restored the impairment of IKCa- and SKCa-mediated vasodilatation in mesenteric arteries from streptozotocin-induced type 2 diabetic rats and that from normal rats incubated with advanced glycation end products (AGEs) for 3 hours. In cultured human umbilical vein endothelial cells (HUVECs), 1 μM metformin reversed AGE-induced increase of ROS and attenuated AGE- and H2O2- induced downregulation of IKCa and SKCa after long-term incubation (&gt;24 hours). Short-term treatment (3 hours) with 1 μM metformin reversed the decrease of IKCa and SKCa currents induced by AGE incubation for 3 hours without changing the channel expression or the AMPK activation in HUVECs. These results are the first to demonstrate that metformin restored IKCa- and SKCa-mediated vasodilatation impaired by AGEs in rat mesenteric artery, in which the upregulation of channel activity and protein expression is likely involved.