TY - JOUR T1 - Transient Receptor Potential Melastatin-3 (TRPM3)–Induced Activation of AP-1 Requires Ca<sup>2+</sup> Ions and the Transcription Factors c-Jun, ATF2, and Ternary Complex Factor JF - Molecular Pharmacology JO - Mol Pharmacol SP - 617 LP - 628 DO - 10.1124/mol.114.095695 VL - 87 IS - 4 AU - Andrea Lesch AU - Xin Hui AU - Peter Lipp AU - Gerald Thiel Y1 - 2015/04/01 UR - http://molpharm.aspetjournals.org/content/87/4/617.abstract N2 - The steroid pregnenolone sulfate activates the transcription factor activator protein-1 (AP-1) via stimulation of transient receptor potential melastatin-3 (TRPM3) channels. Here, we show that the signaling pathway requires an influx of Ca2+ ions into the cells and a rise in the intracellular Ca2+ levels. The upregulation of AP-1 was attenuated in cells that overexpressed mitogen activated protein kinase phosphatase–1, indicating that Ca2+ ions prolong the signaling cascade via activation of mitogen activated protein kinases. On the transcriptional level, expression of a dominant-negative mutant of the basic region leucine zipper protein c-Jun, a major constituent of the AP-1 transcription factor complex, or expression of a c-Jun–specific short hairpin RNA attenuated pregnenolone sulfate–induced AP-1 activation. In addition, stimulation of TRPM3 channels increased the transcriptional activation potential of the basic region leucine zipper protein ATF2. Inhibition of ATF2 target gene expression via expression of a dominant-negative mutant of ATF2 or expression of an ATF2-specific short hairpin RNA interfered with TRPM3-mediated stimulation of AP-1. Moreover, we show that a dominant-negative mutant of the ternary complex factor (TCF) Elk-1 attenuated the upregulation of AP-1 following stimulation of TRPM3 channels. Thus, c-Jun, ATF2, and TCFs are required to connect the intracellular signaling cascade elicited by activation of TRPM3 channels with enhanced transcription of AP-1–regulated genes. We conclude that pregnenolone sulfate–induced TRPM3 channel activation changes the gene expression pattern of the cells by activating transcription of c-Jun-, ATF2-, and TCF-controlled genes. ER -