TY - JOUR T1 - G Protein–Coupled Receptor Multimers: A Question Still Open Despite the Use of Novel Approaches JF - Molecular Pharmacology JO - Mol Pharmacol SP - 561 LP - 571 DO - 10.1124/mol.115.099440 VL - 88 IS - 3 AU - Henry F. Vischer AU - Marián Castro AU - Jean-Philippe Pin Y1 - 2015/09/01 UR - http://molpharm.aspetjournals.org/content/88/3/561.abstract N2 - Heteromerization of G protein–coupled receptors (GPCRs) can significantly change the functional properties of involved receptors. Various biochemical and biophysical methodologies have been developed in the last two decades to identify and functionally evaluate GPCR heteromers in heterologous cells, with recent approaches focusing on GPCR complex stoichiometry and stability. Yet validation of these observations in native tissues is still lagging behind for the majority of GPCR heteromers. Remarkably, recent studies, particularly some involving advanced fluorescence microscopy techniques, are contributing to our current knowledge of aspects that were not well known until now, such as GPCR complex stoichiometry and stability. In parallel, a growing effort is being applied to move the field forward into native systems. This short review will highlight recent developments to study the stoichiometry and stability of GPCR complexes and methodologies to detect native GPCR dimers. ER -