@article {Wellsmol.112.080507, author = {Christopher Alexander Wells and Zack P. Zurawski and Katherine M. Betke and Yun Young Yim and Karren Hyde and Shelagh Rodriguez and Simon Alford and Heidi E. Hamm}, title = {Gβγ Inhibits Exocytosis Via Interaction with Critical Residues on SNAP-25}, elocation-id = {mol.112.080507}, year = {2012}, doi = {10.1124/mol.112.080507}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Spatial and temporal regulation of neurotransmitter release is a complex process accomplished by the exocytotic machinery working in tandem with numerous regulatory proteins. G-protein βγ dimers regulate the core process of exocytosis by interacting with the SNARE proteins SNAP-25, syntaxin 1A, and synaptobrevin. Gβγ binding to ternary SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptors) overlaps with synaptotagmin{\textquoteright}s calcium-dependent binding, inhibiting synaptotagmin-1 binding and fusion of the synaptic vesicle. To further explore the binding sites of Gβγ on SNAP-25, peptides based on the sequence of SNAP-25 were screened for Gβγ binding. Peptides that bound Gβγ were subjected to alanine scanning mutagenesis to determine their relevance to the Gβγ{\textendash}SNAP-25 interaction. Peptides from this screen were tested in protein-protein interaction assays for their ability to modulate the interaction of Gβγ with SNAP-25. A peptide from the C-terminus, residues 193-206, significantly inhibited the interaction. Additionally, Ala mutants of SNAP-25 residues from the C-terminus of SNAP-25, as well as from the amino terminal region decreased binding to Gβ1γ1. When SNAP-25 with 8 residues mutated to alanine was assembled with syntaxin 1A, there was significantly reduced affinity of this mutated t-SNARE for Gβγ, but it still interacted with synaptotagmin-1 in a Ca2+-dependent manner and reconstituted evoked exocytosis in BoNT/E-treated neurons. However, the mutant SNAP-25 could no longer support 5-HT-mediated inhibition of exocytosis.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/early/2012/09/07/mol.112.080507}, eprint = {https://molpharm.aspetjournals.org/content/early/2012/09/07/mol.112.080507.full.pdf}, journal = {Molecular Pharmacology} }