TY - JOUR T1 - Studies on an Acetylcholine Binding Protein Identify a Basic Residue in Loop G on the β1-strand as a New Structural Determinant of Neonicotinoid Actions JF - Molecular Pharmacology JO - Mol Pharmacol DO - 10.1124/mol.114.094698 SP - mol.114.094698 AU - Makoto Ihara AU - Toshihide Okajima AU - Atsuko Yamashita AU - Takuma Oda AU - Takuya Asano AU - Mikana Matsui AU - David B. Sattelle AU - Kazuhiko Matsuda Y1 - 2014/01/01 UR - http://molpharm.aspetjournals.org/content/early/2014/09/29/mol.114.094698.abstract N2 - Neonicotinoid insecticides target insect nicotinic acetylcholine receptors (nAChRs). Their widespread use and possible risks to pollinators make it extremely urgent to understand the mechanisms underlying their actions on insect nAChRs. We therefore elucidated X-ray crystal structures of the Lymnaea stagnalis acetylcholine binding protein (Ls-AChBP) and its Gln55Arg mutant, more closely resembling insect nAChRs, in complex with a nitromethylene imidacloprid analogue (CH-IMI) and desnitro imidacloprid metabolite (DN-IMI) as well as commercial neonicotinoids, imidacloprid, clothianidin and thiacloprid. Unlike imidacloprid, clothianidin and CH-IMI, thiacloprid did not stack with Tyr185 in the wild-type Ls-AChBP, but did in the Gln55Arg mutant, interacting electrostatically with Arg55. In contrast, DN-IMI lacking the NO2 group was directed away from Lys34 and Arg55 to form hydrogen bonds with Tyr89 in loop A and the main chain carbonyl of Trp143 in loop B. Unexpectedly, we found that several neonicotinoids interacted with Lys34 in loop G on the β1 strand in the crystal structure of the Gln55Arg mutant. Basic residues introduced into the α7 nAChR at positions equivalent to AChBP Lys34 and Arg55 enhanced agonist actions of neonicotinoids, while reducing the actions of acetylcholine, (-)-nicotine and DN-IMI. Thus, not only the basic residues in loop D, but also those in loop G determine the actions of neonicotinoids. These novel findings provide new insights into the modes of action of neonicotinoids and emerging derivatives. ER -