RT Journal Article SR Electronic T1 G Protein-Coupled Receptor Multimers: A Question Still Open Despite the Use of Novel Approaches JF Molecular Pharmacology JO Mol Pharmacol FD American Society for Pharmacology and Experimental Therapeutics SP mol.115.099440 DO 10.1124/mol.115.099440 A1 Henry F Vischer A1 Marian Castro A1 Jean-Philippe R. Pin YR 2015 UL http://molpharm.aspetjournals.org/content/early/2015/07/02/mol.115.099440.abstract AB Heteromerization of G protein-coupled receptors (GPCR) can significantly change the functional properties of involved receptors. Various biochemical and biophysical methodologies have been developed the last two decades to identify and functionally evaluate GPCR heteromers in heterologous cells, with recent approaches focusing on GPCR complex stoichiometry and stability. Yet, validation of these observations in native tissues is still lacking behind for the majority of GPCR heteromers. Remarkably, recent studies, particularly some involving advanced fluorescence microscopy techniques, are contributing to our current knowledge on aspects that were not well known till now such as GPCR complex stoichiometry and stability. In parallel, a growing effort is being applied to move the field forward into native systems. This short review will highlight recent developments to study stoichiometry and stability of GPCR complexes and methodologies to detect native GPCR dimers.