TY - JOUR T1 - Transforming Growth Factor-<em>β</em>1/Activin Receptor-like Kinase 5-Mediated Cell Migration is Dependent on the Protein Proteinase-Activated Receptor 2 but not on Proteinase-Activated Receptor 2-Stimulated G<sub>q</sub>-Calcium Signaling JF - Molecular Pharmacology JO - Mol Pharmacol SP - 519 LP - 532 DO - 10.1124/mol.117.109017 VL - 92 IS - 5 AU - Hendrik Ungefroren AU - David Witte AU - Koichiro Mihara AU - Bernhard H. Rauch AU - Petra Henklein AU - Olaf Jöhren AU - Shirin Bonni AU - Utz Settmacher AU - Hendrik Lehnert AU - Morley D. Hollenberg AU - Roland Kaufmann AU - Frank Gieseler Y1 - 2017/11/01 UR - http://molpharm.aspetjournals.org/content/92/5/519.abstract N2 - Transforming growth factor-β (TGF-β), serine proteinases such as trypsin, and proteinase-activated receptor 2 (PAR2) promote tumor development by stimulating invasion and metastasis. Previously, we found that in cancer cells derived from pancreatic ductal adenocarcinoma (PDAC) PAR2 protein is necessary for TGF-β1-dependent cell motility. Here, we show in the same cells that, conversely, the type I TGF-β receptor activin receptor-like kinase 5 is dispensable for trypsin and PAR2 activating peptide (PAR2-AP)-induced migration. To reveal whether Gq-calcium signaling is a prerequisite for PAR2 to enhance TGF-β signaling, we investigated the effects of PAR2-APs, PAR2 mutation and PAR2 inhibitors on TGF-β1-induced migration, reporter gene activity, and Smad activation. Stimulation of cells with PAR2-AP alone failed to enhance basal or TGF-β1-induced C-terminal phosphorylation of Smad3, Smad-dependent activity of a luciferase reporter gene, and cell migration. Consistently, in complementary loss of function studies, abrogation of the PAR2-Gq-calcium signaling arm failed to suppress TGF-β1-induced cell migration, reporter gene activity, and Smad3 activation. Together, our findings suggest that the calcium-regulating motif is not required for PAR2 to synergize with TGF-β1 to promote cell motility. Additional experiments in PDAC cells revealed that PAR2 and TGF-β1 synergy may involve TGF-β1 induction of enzymes that cause autocrine cleavage/activation of PAR2, possibly through a biased signaling function. Our results suggest that although reducing PAR2 protein expression may potentially block TGF-β’s prooncogenic function, inhibiting PAR2-Gq-calcium signaling alone would not be sufficient to achieve this effect. ER -