FFA2 mutant receptor expression per 5 μg of protein as determined by eYFP fluorescence
Values are presented as mean ± S.E.M.
| FFA2 Receptor | eYFP | n |
|---|---|---|
| RLU | ||
| HEK293 no receptor | 556 ± 25.9 | |
| Wild type | 4193 ± 405 | 4 |
| H140A (4.56) | 2110 ± 320 | 4 |
| L173A (ECL2) | 2041 ± 431 | 3 |
| R180A (5.39) | 1798 ± 187 | 6 |
| H242A (6.55) | 2655 ± 101 | 5 |
| R255A (7.35) | 3024 ± 201 | 5 |
| R180A/R255A (5.39/7.35) | 2072 ± 101 | 3 |
| FFA2 (ECL2 FFA3) | 1886 ± 112 | 3 |
RLU, relative light unit; HEK, human embryonic kidney.