TABLE 2

Abl kinase inhibition with STI571 alters α3*-nAChR mediated synaptic transmission

Spontaneous and evoked synaptic activity was recorded from neurons in 4-day CG cultures. F_s and A_s indicate the frequency and amplitude of sEPSCs. mEPSCs were acquired by blocking impulses with 1 μM TTX. mEPSC frequency and amplitude are indicated by f_s and a_s, respectively, f_s* indicating normalized mEPSC frequency. Quantal content (m) determinations were made based on spontaneous (m_s) and evoked EPSCs (m_e and m_f) as described under Results. Values from neurons treated with STI571 (10 μM) for 1 and 24 h have been combined.

	sEPSC		mEPSC			Quantal Content
	F_s	A_s	f_s	f_s^*	a_s	m_s	m_e	m_f
	Hz	pA	Hz		pA
Control	0.83 ± 0.12 (35)	−27.8 ± 1.5 (35)	0.30 ± 0.11 (19)	1.00 ± 0.13 (19)	−13.4 ± 1.3 (19)	1.9 ± 0.1 (18)	1.7 ± 0.4 (6)	1.5 ± 0.3 (6)
STI571	0.28 ± 0.05 (16)^*	−11.9 ± 1.3 (16)^*	0.10 ± 0.01 (20)	0.95 ± 0.18 (20)	−6.1 ± 0.1 (20)^*	1.9 ± 0.2 (16)	2.3 ± 0.4 (6)	1.3 ± 0.3 (6)
p =	<0.0001	<0.0001	0.06	0.82	<0.0001	0.99	0.27	0.65

↵* Parameter values in the STI571-treated group that are significantly different from those in sham-treated controls (P < 0.05, Student's unpaired two-tailed t test).