Effect of Vinca alkaloids at the IC concentration for mitotic block on spindle length in HeLa cells
| Drug (IC50 value) | Pole-to-Pole Distance |
|---|---|
| μm | |
| No drug (control) | 9.7 ± 0.3 |
| Vinflunine (38 nM) | 7.8 ± 0.3 |
| Vinorelbine (3.8 nM) | 6.9 ± 0.1 |
| Vinblastine (1.1 nM) | 7.6 ± 0.1 |
Spindle length was measured as the interpolar distance, namely the distance between centrosomes at opposite spindle poles of bipolar spindles after fixing and staining cells with tubulin antibodies (mean ± S.E.M. of at least 50 spindles from two independent experiments for each test condition).