Table 1

Kinetic studies of microsomal GST activation by ONOO⁻

Condition	K_cat	K_m	K_cat/K_m
	s ⁻¹	mM	s⁻¹ mM ⁻¹
Control	1.6 ± 0.1	2.6 ± 0.2	0.59
ONOO⁻	11 ± 2.5^1-160	12 ± 3.2^1-160	1.67

Purified enzyme (20 μg/ml) was incubated with 2.0 mM ONOO⁻ for 10 s at room temperature. GST activity was measured using 1.0 mM CDNB and varying concentrations of GSH (0.1–1.0 mM) (values represent the mean ± S.D., n = 6).

↵1-160 , P < 0.001 vs control, Student'st test for unpaired data.