Binding experiments with [3H]GBC and [3H]P1075 in membranes
| [3H]GBC | [3H]P1075 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| GBC | P1075 | P1075 | GBC | |||||||
| KD | n | Ki | A | n | KD | n | Ki | A | n | |
| nM | nM | %B S | nM | nM | %B S | |||||
| SUR2B2B(Y1206S) | 3.4 (2.9,3.9) | 4 | 5.6 (3.4,9.3) | 45 ± 4 | 6 | 6.5 (6.2,6.8) | 3 | 245 (162,372) | 100 | 3 |
| 660 (480,910) | 55 ± 4 | |||||||||
| Kir6.1/SUR2B(Y1206S) | 2.5 (2.2,2.9) | 3 | 5.9 (4.7,7.4) | 73 ± 22-a | 3 | 6.8 (5.6,8.1) | 3 | 2.7 (1.9,3.7)2-b | 59 ± 12-b | 6 |
| 690 (600,790) | 27 ± 22-a | 360 (230,540)2-b | 41 ± 12-b | |||||||
| Kir6.2/SUR2B(Y1206S) | 2.7 (2.3,3.0) | 3 | 7.1 (6.5,7.8) | 75 ± 22-a | 3 | 7.2 (4.4,12.0) | 3 | 4.4 (2.8,6.9) | 75 ± 3 | 4 |
| 650 (340,1230) | 25 ± 22-a | 288 (191,437) | 25 ± 3 | |||||||
| SUR2B | N.D. | N.D. | 3.3 (2.4,4.6) | 4 | 2400 (1900,3000) | 100 | 4 | |||
| Kir6.1/SUR2B | N.D. | N.D. | 4.4 | 72 (50,105) | 62 ± 3 | 3 | ||||
| 2400 (2000,2900) | 38 ± 3 | |||||||||
| Kir6.2/SUR2B | N.D. | N.D. | 4.4 | 50 (21,120) | 42 ± 3 | 3 | ||||
| 200 (1000,4000) | 58 ± 3 | |||||||||
K D/K i values are geometric means from n independent experiments; A denotes the amplitudes in percentage of specific binding. Experiments were performed as described in Fig. 5. Experiments on SUR2B are from Hambrock et al. (1998).