Table 2

Summaries of changes in the drug resistance profiles of the MSTC variants

Cell LinePaclitaxelVincristineDaunomycinDexamethasonePuromycinColchicineMutation
MSTC-2NC ++ ++ + +++ + L865F
MSTC-8▾▾NCNCNC +++ A933T
MSTC-14▾▾NC ++ NC +++ + I862F
MSTC-20NCNCNC▾▾NC +++ +++ L338F
MSTC-21.10▾▾▾ + NCNC +++ +++ N350I
MSTC-24NC + NC +++ + L868W
24TCTP-6▾▾NC +++ +++ ++ NCL868W
N988D

Changes in drug resistance were evaluated by measuring the IC50values for the individual drugs in the W7TB, MS23, and variant cell lines. The change in drug resistance was calculated according to the equation: Embedded Image where Change ≥0.5 = +, ≥1.0 = ++, ≥1.5 = +++, ≥2.0+++ +, ≤−0.33 = ▾, ≤−0.67 = ▾▾, and ≤−1.0 = ▾▾▾. NC indicates no change, or changes smaller than those indicated above, in the drug resistance.