Effect of deletion of amino acids 225–353 of the third intracellular loop of the m1 mAChR on the phosphoinositide response to acetylcholine mediated by the Asp122 and Tyr124 mutants
| Mutant | pEC50 | Emax |
|---|---|---|
| −log (m) | % basal | |
| dLoop | 6.4 ± 0.15-a | 240 ± 60 |
| D122E dLoop | 6.2 ± 0.6 | 250 ± 80 |
| D122N dLoop | 5.9 ± 0.3 | 210 ± 90 |
| D122H dLoop | 5.5 ± 0.2 | 230 ± 80 |
| D122A dLoop | 5.7 ± 0.1 | 170 ± 40 |
| D122S dLoop | 5.9 ± 0.1 | 180 ± 40 |
| D122L dLoop | 4.2 ± 0.2 | 140 ± 50 |
| Y124W dLoop | 6.1 ± 0.2 | 230 ± 60 |
| Y124H dLoop | 5.8 ± 0.1 | 200 ± 30 |
| Y124M dLoop | 5.7 ± 0.3 | 230 ± 40 |
| Y124C dLoop | 6 | 260 |
↵5-a Values are mean ± standard error of three or more experiments.