Quantitative estimates of coimmunoprecipitation of NR1, NR2A, and NR2B subunits with specific antibodies
| Immunoprecipitated with | NR1 | NR2A | NR2B |
|---|---|---|---|
| % Precipitation | |||
| NR1 probed | 96 ± 2 | 56 ± 7 | 75 ± 5 |
| NR2A probed | 76 ± 4 | 97 ± 1 | 59 ± 8 |
| NR2B probed | 84 ± 4 | 48 ± 9 | 94 ± 2 |
Four experiments were performed. Antibodies indicated at the top were used for immunoprecipitation. Antibodies indicated at the left were used to probe the resulting blots. When no measurable signal could be detected in a supernatant (e.g., S in Fig. 3) lane, the estimated limit of sensitivity of the method (98%) was used in the calculation of the mean ± standard error.