Scatchard analysis of basal and DAMGO-stimulated [35S]GTPγS binding to mMOR-CHO cell membranes
| High affinity | Low affinity | |||
|---|---|---|---|---|
| KD | Bmax | KD | Bmax | |
| nm | pmol/mg | nm | pmol/mg | |
| Basal | 15.0 ± 1.2 | 3.4 ± 0.3 | 1128 ± 86 | 449 ± 46 |
| DAMGO | 1.7 ± 0.13-a | 5.5 ± 0.23-a | 1162 ± 88 | 518 ± 36 |
| Net DAMGO | 1.6 ± 0.13-a | 4.6 ± 0.33-a | N.A. | N.A. |
Membranes were incubated with 0.01 nm[35S]GTPγS and 10 μm GDP in the presence and absence of 1 μm DAMGO. Data are mean ± standard error of KD and B maxvalues from biphasic Scatchard analysis of absolute [35S]GTPγS binding and from monophasic Scatchard analysis of net DAMGO-stimulated [35S]GTPγS binding.
N.A., not applicable.
↵3-a , p < 0.01, different from basal.