Induction of reversible protein/DNA complexes in HeLa S3 cells by saintopin-type antibiotics
| Compound | Radioactivity precipitated by K/SDS procedure with increasing drug concentrations | ||||
|---|---|---|---|---|---|
| 0 μm | 0.01 μm | 0.1 μm | 1 μm | 10 μm | |
| % | |||||
| Camptothecin | 2.1 | 5.0 | 10.8 | 23.6 | 34.5 |
| (2.4)1-a | (3.5)1-a | (3.8)1-a | |||
| (4.9)1-b | (5.3)1-b | ||||
| UCE6 | 2.1 | 4.5 | 14.9 | 33.3 | 31.2 |
| (3.0)1-a | (4.0)1-a | (4.2)1-a | |||
| (5.3)1-b | (5.5)1-b | ||||
| Saintopin | 2.1 | 2.2 | 3.3 | 8.6 | 17.0 |
| (3.0)1-a | (2.1)1-a | (3.0)1-a | |||
| (2.1)1-b | (4.2)1-b | ||||
| Saintopin E | 2.1 | 2.7 | 2.9 | 4.3 | 7.2 |
| (3.0)1-a | (1.7)1-a | (2.3)1-a | |||
| (1.8)1-b | (2.8)1-b | ||||
| UCE1022 | 2.1 | 2.1 | 2.1 | 3.2 | 13.9 |
| (2.0)1-a | (2.0)1-a | (2.5)1-a | |||
| (1.6)1-b | (3.0)1-b | ||||
Protein/DNA complexes in drug-treated cells were measured by using the potassium/SDS precipitation method as described in Materals and Methods. The total trichloroacetic acid-precipitable cpm for each sample average was ∼4.5 × 104.