Table 2

Summary of results for differentially regulated mRNAs of chick embryo liver after a 48-hr treatment with phenobarbital

Fragment	Primers^2-a	Length^2-b	Identity^2-c
		bp
Up-regulated PCR Fragments
1	11 /GA	380	Retinal glutamine synthetase
3	13 /GA	150	Unknown
4	6 /CA	280	GST
5	6 /AA	280	GST
6	16 /GA	170	Unknown
10	18 /AT	220	Unknown
12	18 /GT	125	UDP-gluc^2-d
13	6 /GA	280	GST
14	13 /GT	230	New
16	3 /AT	230	Elongation factor 1δ^2-d
18	5 /CA	210	Apolipoprotein B
19	9 /GA	145	Unknown
20	14 /CA	145	New
24	20 /CA	220	ND
26	4 /AT	200	ND
27	13 /AT	230	ND
28	14 /AT	145	ND
29	3 /CT	360	ND
Down-regulated PCR Fragments
2	13 /GA	280	Fibrinogen β-cain
7	17 /CT	400	Complement factor H^2-d
8	16 /AT	150	Unknown
9	18 /AT	230	Unknown
11	20 /AT	230	Unknown
15	9 /AA	180	New
17	8 /CT	260	Fibrinogen γ-chain^2-d
21	13 /CT	280	ND
22	14 /CT	140	ND
23	24 /AA	420	ND
25	22 /CA	270	ND

↵2-a Numbering of random 5′ primers according to Table 1. Oligo-dTMN primers are indicated by their two specific bases at their 3′ end as (ultimate and penultimate positions).
↵2-b The range of bands to be selected has been set between 100 and 500 bp because of the resolution of the sequencing gel used for separating the DDRT-PCR products (Fig. 1).
↵2-c Sequence homologies determined by using FASTA search of GenEMBL database (August 1996).
↵2-d Sequences unknown in chicken but showing homology to sequences in other species (for details, see text).
New, new sequence, cDNA isolated; Unknown, new sequence, cDNA not isolated; ND, not further analyzed.