Time of application of 3 × 10−5m DMPP | Pretreatment (t = 0 sec) | Agonist-induced [Ca2+]i | Control |
---|---|---|---|
% | |||
20 sec | Buffer | 65 ± 8 | 100 |
Subthreshold DMPP | 80 ± 10 | 123 | |
40 sec | Buffer | 57 ± 4 | 100 |
Subthreshold DMPP | 93 ± 8* | 163 | |
60 sec | Buffer | 60 ± 2 | 100 |
Subthreshold DMPP | 92 ± 7* | 153 | |
80 sec | Buffer | 55 ± 6 | 100 |
Subthreshold DMPP | 73 ± 5 | 130 | |
120 sec | Buffer | 49 ± 9 | 100 |
Subthreshold DMPP | 51 ± 8 | 104 | |
160 sec | Buffer | 58 ± 4 | 100 |
Subthreshold DMPP | 64 ± 12 | 109 |
Dispersed cells (1–2 × 106) were exposed to subthreshold concentrations of DMPP or buffer followed 20–160 sec later by exposure to a maximal concentration of DMPP (3 × 10−5m). The values represent the mean ± standard error of four to six determinations from two experiments. Significance of difference from control: *p < 0.01.