Apparent kinetic constants for cysteine conjugateS-oxidation by rabbit liver microsomes
| Substrate | Vmax | Km | Vmax/Km |
|---|---|---|---|
| nmol/mg protein·min | mm | ||
| SAC | 3.0 | 3.6 | 0.84 |
| SBC | 6.6 | 18.0 | 0.37 |
| TCVC | 0.1 | 4.8 | 0.02 |
| DCVC | 0.7 | 86.0 | 0.01 |
Kinetic constants were determined as described in Experimental Procedures using double-reciprocal plots. All r values > 0.98. Incubations contained 2 mm NADPH, 0.5–1.0 mg of rabbit liver microsomal protein, and the following concentrations of substrate: 1–10 mm for TCVC and SBC, 1–30 mmfor SAC, and 10–30 mm for DCVC.
Data represent the mean of three experiments, except SAC, which is the mean of four experiments.