Treatment | Specific binding | ||
---|---|---|---|
Experiment I | Experiment II | Experiment III | |
fmol/mg protein | |||
Control | 88.3 ± 2.6 | 203 ± 11 | 590 ± 111 |
NE (0.1 μm) | 96.0 ± 6.2 | 258 ± 48 | 656 ± 105 |
Isoproterenol (0.1 μm) | n.d. | 181 ± 102 | 595 ± 124 |
Phenylephrine (1 μm) | n.d. | 150 ± 67 | 453 ± 104 |
Forskolin (100 μg/ml) | 118 ± 111-a | 389 ± 261-a | n.d. |
VIP (0.1 μm) | 92.0 ± 8.4 | n.d. | n.d. |
Pineal glands were treated in culture for the indicated times with the drugs. Experiment I: Treatment for 12 hr, binding determined on seven to eight individual glands using 50 pM 125I-HEAT.Experiment II: Treatment for 24 hr, binding determined on two to three pairs of glands using 1 nm[3H]prazosin. Experiment III: Treatment for 24 hr, binding determined on four to five pairs of glands using 1 nm [3H]prazosin. Data are presented as the mean ± standard error, and statistical significance was tested using one-way ANOVA within each experiment.
↵1-a p < 0.05 compared with control; n.d., not determined.