Comparison of the specific enzyme activities from rat liver homogenate and the cell supernatants of the recombinant protein from E. coli
| Total protein | Specific activity | |
|---|---|---|
| mg/ml | nmol/min/mg | |
| Crude liver homogenate | 2.94 | 0.88 |
| Purified cytosolic epimerase from rat liver | 0.005 | 3710 |
| M15-PQE30 control (without IPTG induction) | 10.0 | — |
| Recombinant protein (60 min after IPTG induction) | 10.0 | 2.6 |