Identification of putative NREs in promotor of AHR and CYP1A1 genes
| NRE | CYP1A1 | AHR | TGF-β2-a |
|---|---|---|---|
| Rat Transin2-b | 5′-GNNTTGGNGA | 5′-GNNTTGGNGA | + |
| nt −1447 to −1438 | nt −1433 to −1424 | ||
| nt −127 to −118 | |||
| Human IL-22-c | 5′-TGTcaAAaATGcAAA | 5′-TGatTcAaATGTAcAt | + |
| nt −2632 to −2618 | nt −1151 to −1136 | ||
| Human CYP1A12-d | 5′- GTGCTCTG CCAAT CAAAGCAC | 5′- GTaCTCTG aatAg CAAAGCAC | ? |
| nt −794 to −774 | nt −1046 to −1026 |
AHR (33) and CYP1A1 promoter (34) were screened for known negative regulatory cis-acting elements.
↵2-a TGF-β responsiveness: +, proven; ?, not shown.
↵2-b Consensus sequence for transin/stomelysin TGF-β inhibitory element: GNNTTGGNGA (20).
↵2-c Noncanonical AP-1/Oct-1 binding sites in human IL-2 gene: −87 5′-TGTGTAATATGTAAAA(35). Putative Ap-1 [TGARTC/AA (36)] and Oct-1 binding site [5′-ATGCAAAT (36)] as described for IL-2 NRE are underlined or given in italics, respectively.
↵2-d Palindromic sequences identified in the NRE of human CYP1A1 (37) are double-underlined.
nt, nucleotides.