Cell | EC50 | Fold increase | Basal | Maximal |
---|---|---|---|---|
nm | ×103dpm/well | |||
GRP-R-Low | 1.0 ± 0.1 | 8.2 ± 1.0 | 2.3 ± 0.2 | 17.2 ± 0.7 |
GRP-R-Med | 1.1 ± 0.2 | 24.0 ± 3.1 | 2.8 ± 0.4 | 60.5 ± 4.2 |
GRP-R-Hi | 0.9 ± 0.2 | 57.5 ± 4.1 | 7.2 ± 0.6 | 401.6 ± 21.3 |
Experimental conditions were as described in the 2 legend to Fig. 2. [3H]IP were measured after loading confluent cells withmyo-[2-3H]inositol in DMEM and 2% fetal bovine serum. Values are expressed as the fold increase in total cellular [3H]IP when stimulated with 1 μmbombesin for 60 min at 37°. EC50, effective concentration causing a half-maximal increase in [3H]IP determined using a curve-fitting program (Kaleidograph). Values are given as mean ± standard error of at least four separate experiments.