Table 3

1′-OH-MDZ/4-hydroxymidazolam formation ratios from incubations of MDZ with cultured Caco-2 cells

CellDuration of 1α,25-(OH)2-D3 treatmentExtracellular matrixDuration of MDZ incubation1′-OH-MDZ/4-OH-MDZ
ApicalBasolateral
hr hr
Clone 5
336Matrigel7.55.45.2
UntreatedMatrigel7.54.53.6
Clone 7
12Matrigel63.8N.D.
120Matrigel65.1N.D.
336Matrigel65.3N.D.
336laminin75.45.2
336Matrigel75.35.2

Clone 5 is the clone that maximally expresses CYP3A immunoreactive protein, whether treated or untreated with 1α,25-(OH)2-D3. Clone 7 is the clone that was used for all of the other experiments described.

    • N.D., not done.