Effects of dimaprit and dbcAMP on U-937 cell differentiation
| Chemotaxis assay migrated cells | Cell surface marker-positive cells | NBT-positive cells | ||
|---|---|---|---|---|
| CD14 | CD11b | |||
| % | ||||
| Control | 0.4 ± 0.1 | 6.3 ± 3.3 | 34.9 ± 5.0 | 4.2 ± 2.9 |
| Dimaprit | 1.2 ± 0.1 | 8.5 ± 3.1 | 37.3 ± 3.4 | 3.9 ± 0.1 |
| dbcAMP | 41.0 ± 2.11-a | 25.1 ± 4.51-a | 68.9 ± 1.71-a | 35 ± 3.21-a |
U937 cells were treated for 72 hr with 10 μm dimaprit or 0.4 mm dbcAMP. The proportion of chemoattracted cells, induction of cell surface markers for myeloid differentiation, and NBT-reducing activity were performed as described in Materials and Methods. Data are the mean ± standard error of assay quadruplicates. Similar results were obtained in at least three independent experiments.
↵1-a p < 0.002 relative to control.