Binding characteristics of Ltk− GH4C1 cells expressing transfected wild-type and mutant 5-HT1A receptors
| 5-HT1Areceptor | Bmax | Kd |
|---|---|---|
| pmol/mg of protein | nm | |
| Ltk−: Wild-type | 1.64 ± 0.38 | 2.74 ± 0.38 |
| Ltk−: T343A1-a | 0.62 ± 0.10 | 7.80 ± 2.50 |
| Ltk−: T149A1 | 0.36 ± 0.06 | 5.40 ± 0.30 |
| Ltk−: T149A2 | 0.34 ± 0.09 | 4.30 ± 0.06 |
| GH4: Wild-type | 1.10 ± 0.45 | 5.62 ± 1.50 |
| GH4: T149A | 2.71 ± 0.90 | 3.57 ± 1.10 |
Membranes from clones stably transfected with wild-type or mutant 5-HT1A receptors were prepared and subjected to saturation binding analysis using [3H]DPAT as a selective agonist (see text). Kd and B maxvalues were determined by nonlinear regression analysis of saturation binding data derived with the RECEPTOR FIT (Lundon program). Values shown are the mean ± standard error of data from at least three independent experiments except for Ltk−: T149A2 (two experiments).
↵1-a Data from Ref. 24.