Ligand binding affinity and cell surface expression of V2R in COS.M6 cells
| V2R | Sites/cell | KD |
|---|---|---|
| % wild-type | nm | |
| Wild-type | 100 | 4.7 ± 0.4 |
| CC341,342SS | 57 ± 3 | 4.2 ± 0.3 |
| CC341,342GG | 63 ± 4 | 4.0 ± 0.1 |
| C341S | 110 ± 12 | 4.8 ± 0.5 |
| C342S | 91 ± 11 | 4.5 ± 0.4 |
| G345ter | 49 ± 5 | 4.5 ± 0.5 |
| G345ter (CC-SS) | 16 ± 2 | 3.9 ± 0.4 |
KD and number of sites per cell for the wild-type and mutant V2Rs expressed transiently in COS.M6 cells. The data are expressed as percent of wild-type V2R value. Expression level for the wild-type receptor was 6–10 × 106sites/cell. Data are expressed as the mean ± standard error from four to six experiments.