Oligonucleotide primers used for gene expression analysis by RT-PCR
| Gene | 5′ Primer sequence | 3′ Primer sequence | Size of product | Annealing temperature | Cycles |
|---|---|---|---|---|---|
| bp | °C | ||||
| IL-1α | 5′-CAAAAGGAAGGGGACAATAGC-3′ | 5′-TAAGGATGGGAGGGAAAAAGA-3′ | 418 | 54 | 35 |
| IL-1β | 5′-ATGGCAACTGTTCCTGAACTCAAC-3′ | 5′-CAGGACAGGTATAGATTCTTTCCTTT-3′ | 563 | 55 | 33 |
| IL-2 | 5′-ATGTACAGCATGCAGTCTGCATC-3′ | 5′-GGCTTGTTGAGATGATGCTTTGACA-3′ | 502 | 58 | 35 |
| IL-4 | 5′-ATGGGTCTCAACCCCCAGCTAGT-3′ | 5′-GCTCTTTAGGCTTTCCAGGAAGTC-3′ | 399 | 55 | 35 |
| IL-6 | 5′-ATGAAGTTCCTCTCTGCAAGAGACT-3′ | 5′-CACTAGGTTTGCCGAGTAGATCTC-3′ | 638 | 51 | 35 |
| IL-7 | 5′-GCCTGTCACATCATCTGAGTGCC-3′ | 5′-CAGGAGGCATCCAGGAACTTCTG-3′ | 496 | 58 | 28 |
| TGF-β1 | 5′-CTCCCACTCCCGTGGCTTCTAG-3′ | 5′-GTTCCACATGTTGCTCCACACTTG-3′ | 472 | 55 | 35 |
| TGF-β2 | 5′-AGAATCGTCCGCTTTGATGTC-3′ | 5′-TCTTCGCTTTTATTCGGGATG-3′ | 353 | 56 | 35 |
| TGF-β3 | 5′-TTCGTTTCAATGTGTCCTCAGT-3′ | 5′-GTTGTGGTGATCCTTTTGCTTC-3′ | 431 | 56 | 35 |
| TNF-α | 5′-ATGAGCACAGAAAGCATGATCCGC-3′ | 5′-CCAAAGTAGACCTGCCCGGACTC-3′ | 692 | 60 | 28 |
| IFN-γ | 5′-TGAACGCTACACACTGCATCTTGG-3′ | 5′-CGACTCCTTTTCCGCTTCCTGAG-3′ | 460 | 55 | 40 |
| PAI-2 | 5′-TGGTGCTGGTGAATGCTGTCTAC-3′ | 5′-AAAGGGATGATCGGCCACAAACT-3′ | 596 | 58 | 30 |
| CYP1A1 | 5′-CCCACAGCACCACAAGAGATA-3′ | 5′-AAGTAGGAGGCAGGCACAATGTC-3′ | 499 | 60 | 30 |
| CYP1B1 | 5′-GGCGTTCGGTCACTACTCTG-3′ | 5′-AGGTTGGGCTGGTCACTCAT-3′ | 738 | 60 | 30 |
| Ah-R | 5′-ACATAACGGACGAAATCCTGACC-3′ | 5′-TCAACTCTGCACCTTGCTTAGGA-3′ | 710 | 60 | 30 |
| ARNT | 5′-GCTATAATCATTCCCAGGTTTCT-3′ | 5′-CATTGTTGTAGGTGTTGCTTTGG-3′ | 768 | 60 | 30 |
| HPRT | 5′-GCTGGTGAAAAGGACCTCT-3′ | 5′-CACAGGACTAGAACACCTGC-3′ | 249 | Same as the target gene | 25 |