Table 1

Inhibition of polymerase-mediated DNA chain elongation by diphosphoryl ANPs

DrugIC50
Pol αPol δ
μm
PMEGpp6.02.5
PMEApp90.019.0

The IC50 values for each polymerase were measured in assays contained 0.5 μM DNA, 5 μM dNTPs and various concentrations of compounds. In pol δ experiments PCNA was added as described in Materials and Methods.