Saturation and competition binding assay data for wild-type and Y348A α1B-ARs
| Wild-type | Y348A | |
|---|---|---|
| Saturation assays | ||
| Kd for [3H]prazosin (pm) | 41 ± 2 (4, 3) | 44 ± 2 (5, 3) |
| B max range (fmol/mg of protein) | 150–440 (4, 3) | 60–530 (5, 3) |
| Competition assays | ||
| Ki for prazosin (pm) | 46 ± 2 (6, 5) | 61 ± 4 (4, 4) |
| Ki for epinephrine (μm) | 2.0 ± 0.2 (7, 5) | 0.18 ± 0.02 (5, 4) |
Values in parentheses are the number of experiments and the number of different clones, respectively.