Treatment | Nitrite |
---|---|
nmol/200 islets/24 hr | |
Control | 1.3 ± 0.4 |
IL-1β (5 units/ml) | 4.5 ± 0.3 |
IL-1β + 1 (5 units/ml) | 4.5 ± 0.3 |
IL-1β + 1 mm aspirin | 3.6 ± 0.6 |
IL-1β + 3 mm aspirin | 3.1 ± 0.4 |
IL-1β + 5 mm aspirin | 2.6 ± 0.5 |
IL-1β + 10 mmaspirin | 1.2 ± 0.3 |
IL-1β + 20 mmaspirin | 0.8 ± 0.2 |
IL-1β + NMMA (0.5 mm) | 1.2 ± 0.4 |
Rat islets (200) were cultured at 37° for 24 hr in 500 μl of CMRL-1066 in the presence and absence of IL-1β (5 units/ml) or IL-1β plus various concentrations of 1–20 mm aspirin. As a control, 0.5 mm NMMA, a competitive inhibitor of NOS, was incubated with IL-1β. The culture medium was removed, and 50-μl aliquots were mixed with 50 μl of Griess reagent. Nitrite production was determined at an absorbance of 540 nm using a Titertek Multiskan MCC/340 plate reader. Results are the average ± standard error of six individual experiments containing three replicates in each experiment.