[3H]CPX binding | Bmax | Kd |
---|---|---|
fmol/mg of protein | nm | |
Pretreatment | ||
Control | 275 ± 14 | 0.57 ± 0.06 |
CPA | 262 ± 16 | 0.57 ± 0.02 |
p-DITC-ADAC | 153 ± 111-a | 0.56 ± 0.02 |
DDT cells were incubated with 1 μm p-DITC-ADAC or 1 μm CPA for 1 hr at 37° followed by six washes with HBSS. Cell membranes were then prepared, and the A1-AdoRs were determined by [3H]CPX (0.125–4 nm) binding to DDT cell membranes. The Kd andB max values were derived from Rosenthal plots. Data are the mean ± standard error of four or five separate determinations.
↵1-a p < 0.0005 versus control.