Skeletal muscle membrane fraction | Specific [3H]P1075 binding | Na+/K+ATPase | Ca2+ATPase | Azidesensitive ATPase | Cholesterol |
---|---|---|---|---|---|
fmol/mg of protein | μmol/hr/mg of protein | ||||
P1 | 44 ± 30 | 0 | 262 | 14.7 | 0.03 |
P2 | 38 ± 40 | 0.3 | 6.7 | 11.1 | 0.04 |
P3 | 160 ± 12 | 1.1 | 925 | 24.4 | 0.08 |
T tubules | 309 ± 15 | 5.3 | 8.3 | 49 | 0.52 |
Membrane fractions were prepared as described in Materials and Methods, and the stated enzyme activities and cholesterol levels were determined. [3H]P1075 binding was determined using 14 nm. Values are mean ± standard error of two or three determinations.