Detachment of HT29, Neo, ts29-A, and ts29-G cells maintained at 38° or 32° for 72 hr.
| Cell Line | Detached cells (38°) | Detached cells (32°) |
|---|---|---|
| % | ||
| HT29 | 2.31, 1.59 | 1.69, 0.78 |
| Neo | 2.92, 2.98 | 2.58, 1.43 |
| ts29-A | 3.47, 4.69 | 4.10, 6.52 |
| ts29-G | 2.13, 3.72 | 4.30, 3.90 |
The percentage of detached cells in samples that grew at either 38° or 32° for 72 hr was determined by separately counting the number of cells in the sample media and the number of trypsinized cells that had been attached to the dish. Values shown are single determinations from two separate experiments.