Treatment | Concentration1-b | Nitrite (μM) |
---|---|---|
None | 12 ± 51-c | |
TNFα/LPS/ethanol1-a | 47 ± 8 | |
+ NAC | 5 mM | 43 ± 6 |
+ Ascorbic acid | 100 μM | 49 ± 10 |
+αTocopherol | 25 μM | 45 ± 9 |
+Curcumin | 10 μM | 51 ± 11 |
+Staurosporine | 1 μM | 45 ± 3 |
+Herbimycin A | 5 μM | 11 ± 3* |
+Genistein | 20 μM | 42 ± 10 |
+ N-nitro-L-arginine | 100 μM | 13 ± 4* |
↵1-a Hepatocytes were incubated with TNFα (1 ng/ml)/LPS (1 μg/ml)/ethanol (1 mM) or plus above modulators for 24 h. Media samples were collected and assayed for total nitrite and nitrate accumulation.
↵1-b The concentration of these inhibitors and antioxidants used here was noncytotoxic to hepatocytes under this experimental condition.
↵1-c Values are mean ± standard error of triplicate samples in two independent experiments. *Statistically significant decrease in nitrite production compared with ethanol/TNF-α/LPS-treated cells (P < 0.05).