Condition | AC activity | |
---|---|---|
Vehicle treated | NECA treated | |
pmol/min/mg | ||
5 μm GTP | 1.30 ± 0.15 (5) | 2.85 ± 0.52 (5)2-a |
5 μm Forskolin + 5 μm GTP | 16.00 ± 1.55 (4) | 29.02 ± 4.25 (4)2-a |
5 μm Forskolin + 5 mmMnCl2 | 85.25 ± 2.70 (4) | 84.61 ± 3.00 (4) |
Inhibition by 1 μm IB-MECA (%) | 52 ± 7 (5) | 17 ± 7 (5)2-a |
CHO cells expressing the human A3AR were treated in the absence (Control) or presence (Treated) of 10 μm NECA for 20 hr at 37° before membrane preparation and assay of AC activity in the presence of the indicated compounds as described in Experimental Procedures.
↵2-a Significant (p< 0.05) difference from the activity obtained in membranes from vehicle-treated cells for the number of experiments given in parentheses.