Addition | Viable cells | |
---|---|---|
Control | Menadione | |
% | ||
Experiment 1 | ||
Control | 54 ± 3.8 | 85 ± 3.6 |
Cycloheximide | 44 ± 3.3 | 49 ± 4.5 |
NAC | 55 ± 3.1 | 61 ± 4.5 |
Experiment 2 | ||
Control | 28 ± 2.0 | 46 ± 2.7 |
PDTC | 24 ± 2.0 | 28 ± 0.8 |
Thiourea | 26 ± 5.6 | 27 ± 5.1 |
Uric acid | 31 ± 6.6 | 26 ± 6.7 |
Hep G2 cells were pretreated with control MEM or 3 μmmenadione for 45 min in the presence of the indicated additions. Final concentrations of the additions were 20 μg/ml cycloheximide, 10 mm NAC, 50 μm pyrrolidine dithiocarbamate, 5 mm thiourea, and 0.5 mm uric acid. The cells were then exposed to 18 μm menadione for 18 hr, and viability was determined as described in Materials and Methods. Results are mean ± standard deviation of three to five wells per experiment from three separate experiments.