Inhibition of FITC-LPS binding to LPS-binding sites expressed on stimulated BMC
| Inhibitor | C3H/HeOU cells stimulated with | C3H/HeJ cells stimulated with | ||
|---|---|---|---|---|
| LPS | STP | LPS | STP | |
| None | 62.8 ± 0.8 | 69.0 ± 2.6 | 1.9 ± 0.5 | 61.4 ± 1.6 |
| LPS (10 μg/ml) | 1.8 ± 0.3 | 1.5 ± 0.0 | 0.1 ± 0.0 | 0.4 ± 0.1 |
| PS (10 μg/ml) | 63.3 ± 0.5 | 70.5 ± 0.2 | 2.0 ± 0.3 | 57.6 ± 5.4 |
BMC from C3H/HeOU and C3H/HeJ mice were first exposed (24 hr, 37°C) to LPS (10 ng/ml) or STP (5 nm) in CM (400 μl) in the absence of serum. The LPS- and STP-stimulated cells were then equilibrated for 1 hr at 4°C in medium containing 10% FCS, incubated (2 hr, 4°C) with the inhibitor alone, and reincubated (18 hr, 4°C) with FITC-LPS (0.2 μg/ml) in the presence of the inhibitor (10 μg/ml). Data represent the percentage of fluorescent cells determined by FACS analysis. Values represent the mean ± Standard deviation of duplicate determinations.