Table 2

Agonist-induced [3H]inositol phosphate accumulation and calcium mobilization in cells pretreated with (R)-PIA or DPCPX

Pretreatment[3H]Inositol phosphate accumulationCalcium peak induction
EC50EmaxEC50Emax
nm % of Control nm % of Control
None8  ± 2100  ± 911  ± 2100  ± 6
(R)-PIA10  ± 270  ± 102-a 19  ± 765  ± 3b
DPCPX7  ± 1110  ± 715  ± 6101  ± 2

(R)-PIA-induced [3H]inositol phosphate and intracellular calcium accumulation in cells incubated with or without 50 nm (R)-PIA or 50 nm DPCPX (48 hr) was determined as described in Experimental Procedures. Values are mean ± standard error from three separate experiments.

    • 2-a p < 0.05,bp < 0.02 compared with untreated cells.